glycoprotein mag  (Cell Signaling Technology Inc)

Journal: CNS neuroscience & therapeutics

Article Title: Oligodendrocyte Progenitor Cell Transplantation Reduces White Matter Injury in a Fetal Goat Model.

doi: 10.1111/cns.70178

Figure Lengend Snippet: FIGURE 3 | Hypoxic-ischemia (HI) causes decreased myelination and increased neuroinflammation. Tissues were collected at Day 14 after HI. (a) Hematoxylin and eosin staining confirms that the HI group has disordered and loosely arranged white matter in PVWM and SCWM (Scale bar = 500 μm, 20 μm, n = 6 each group). (b) TEM demonstrates that the HI group has a reduced percentage of MNFs and a high g-ratio. Arrows in- dicate myelin sheath (Scale bar = 5 μm, n = 6 each group, Mann–Whitney U test). (c) Immunofluorescent staining displays that the HI group has decreased MBP-positive areas. Arrows represent MBP (Scale bar = 20 μm, n = 6 each group, Student's t-test in PVWM, unpaired t-test with Welch's correction in SCWM). (d) Immunofluorescent staining demonstrates that the HI group has decreased MAG-positive areas. Arrows represent MAG (Scale bar = 20 μm, n = 6 each group, Student's t-test). (e) Immunofluorescent staining reveals that the HI group had increased GFAP-positive ar- eas. Arrows indicate GFAP (Scale bar = 20 μm, n = 6 each group, Student's t-test). (f) Immunofluorescent staining confirms that the HI group has increased Iba-1-positive areas. Arrows represent Iba-1 (Scale bar = 20 μm, n = 6 each group, Student's t-test in PVWM, Mann–Whitney U test in SCWM). *p < 0.05, **p < 0.01, ***p < 0.001. GFAP, glial fibrillary acidic protein; HI, hypoxic ischemia; Iba-1, ionized calcium-binding adaptor mol- ecule-1; MAG, myelin-associated glycoprotein; MBP, myelin basic protein; MNFs, myelinated nerve fibers; PVWM, periventricular white matter; SCWM, subcortical white matter; TEM, transmission electron microscopy.

Article Snippet: Myelin basic protein (MBP) (1:500; Abcam, ab62631) and myelin- associated glycoprotein (MAG) (1:500; Cell Signaling Technology, 9043 s) antibodies were used to detect mature myelin sheaths.

Techniques: Staining, MANN-WHITNEY, Binding Assay, Transmission Assay, Electron Microscopy

Journal: CNS neuroscience & therapeutics

Article Title: Oligodendrocyte Progenitor Cell Transplantation Reduces White Matter Injury in a Fetal Goat Model.

doi: 10.1111/cns.70178

Figure Lengend Snippet: FIGURE 5 | Human oligodendrocyte precursor cells (hOPCs) differentiated into mature oligodendrocytes at Day 21. Immunofluorescent staining showed that transplanted hOPCs expressed markers of mature oligodendrocytes (MBP and MAG) at Day 21. (a) CM-DiI labeled hOPCs (red) bare- ly expressed MBP (green) at Day 14 but strongly expressed MBP at Day 21 both in SCWM and PVWM (Scale bar = 20 μm, n = 6 at each time point. Mann–Whitney U test in PVWM; unpaired t-test with Welch's correction in SCWM) (b) Fluorescence co-localization demonstrates that CM-DiI la- beled hOPCs barely expressed MAG (green) at Day 14 but strongly expressed MAG at Day 21 both in SCWM and PVWM. The arrows indicate hOPCs (Scale bar = 0 μm. n = 6 at each time point, unpaired t-test with Welch's correction). ***p < 0.001. MAG, myelin-associated glycoprotein; MBP, myelin basic protein; PVWM, periventricular white matter; SCWM, subcortical white matter.

Article Snippet: Myelin basic protein (MBP) (1:500; Abcam, ab62631) and myelin- associated glycoprotein (MAG) (1:500; Cell Signaling Technology, 9043 s) antibodies were used to detect mature myelin sheaths.

Techniques: Staining, Labeling, MANN-WHITNEY, Fluorescence